Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Wednesday February 24, 2021

Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6451] to CD2 - BSA and Azide free
Suitable for: Flow Cyt, ICC, WB, IHC-P, IP
Reacts with: Human

Product name

Anti-CD2 antibody [EPR6451] - BSA and Azide free
See all CD2 primary antibodies
Description

Rabbit monoclonal [EPR6451] to CD2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, ICC, WB, IHC-P, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human CD2 aa 200-350. The exact sequence is proprietary.
Database link: P06729
General notes
ab248400 is the carrier-free version of ab131276 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248400 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6451
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

This data was developed using ab131276, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD2 with unpurified ab131276 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Western blot - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

All lanes : Anti-CD2 antibody [EPR6451] (ab131276) at 1/1000 dilution

Lane 1 : Human thymus lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

This data was developed using ab131276, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

This data was developed using ab131276, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab131276 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

This data was developed using ab131276, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labelled with ab131276 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

This data was developed using ab131276, the same antibody clone in a different buffer formulation.ab131276 showing positive staining in Diffuse large B-cell lymphoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

This data was developed using ab131276, the same antibody clone in a different buffer formulation.ab131276 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

This data was developed using ab131276, the same antibody clone in a different buffer formulation.ab131276 showing positive staining in Hodgkin's lymphoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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