Anti-CD19 antibody [BU12] (ab254170)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [BU12] to CD19
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD19 antibody [BU12]
See all CD19 primary antibodies -
Description
Mouse monoclonal [BU12] to CD19 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human -
Immunogen
Tissue, cells or virus within Human CD19. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Flow Cyt: Human peripheral blood mononuclear cell.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
BU12 -
Isotype
IgG1 -
Research areas
Images
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD19 with ab254170 at 1.07 µg/mL, Right compared with a Mouse monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with mouse IgG (Left) or ab254170 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.
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