Anti-Huntingtin antibody (ab155930)
Key features and details
- Rabbit polyclonal to Huntingtin
- Suitable for: ICC/IF, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Huntingtin antibody
See all Huntingtin primary antibodies -
Description
Rabbit polyclonal to Huntingtin -
Host species
Rabbit -
Specificity
Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. Neo-specific antibody ab155930 recognizes the 586 cleaved fragment without detecting the full-length form. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumansELISA Human
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Immunogen
Synthetic peptide:
C-PSDSSEIVLD
conjugated to KLH by a Cysteine residue linker, corresponding to amino acids 575-584 of Human Huntingtin (P42858).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Neoepitope antibodies distinguish smaller cleaved fragments or processed forms of proteins versus the intact full-length or precursor by using a designed peptide purification process to maximize immunoreactivity to a specific cleavage site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA - Anti-Huntingtin antibody (ab155930)Sandwich ELISA was performed with a monoclonal antibody to Huntingtin and ab155930 to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo586 in 293T cells overexpressing the Huntingtin construct.
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Immunocytochemistry/ Immunofluorescence - Anti-Huntingtin antibody (ab155930)Immunofluorescent analysis of formalin fixed, permeabilized 293T cells transfected with Huntingtin23Q (left panel) and Huntingtin148Q (right panel)stop constructs ending in amino acid 586 labeling capase cleaved Huntingtin with ab155930 at 1/50(green). Nuclei were stained with Hoechst 33342 (blue).
