Anti-CD11c antibody [N418] (ab33483)
Key features and details
- Armenian hamster monoclonal [N418] to CD11c
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CD11c antibody [N418]
See all CD11c primary antibodies -
Description
Armenian hamster monoclonal [N418] to CD11c -
Host species
Armenian hamster -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD11c.
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Positive control
- IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
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General notes
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo. -
Clonality
Monoclonal -
Clone number
N418 -
Myeloma
Sp2/0 -
Isotype
IgG -
Research areas
Images
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Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes. -
Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.