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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha

Anti-CD11c antibody [N418] (ab33483)

Price and availability

314 937 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-CD11c antibody [N418] (ab33483)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Armenian hamster monoclonal [N418] to CD11c
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-CD11c antibody [N418]
    See all CD11c primary antibodies
  • Description

    Armenian hamster monoclonal [N418] to CD11c
  • Host species

    Armenian hamster
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD11c.

  • Positive control

    • IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
  • General notes

    Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
  • Clonality

    Monoclonal
  • Clone number

    N418
  • Myeloma

    Sp2/0
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Images

  • Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)
    Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)

    Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488)  preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.


    Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

  • Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)
    Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)

    Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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