Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

50 µl 308 236 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [8A1] to CD105
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG2b

Product name

Anti-CD105 antibody [8A1]
See all CD105 primary antibodies
Description

Mouse monoclonal [8A1] to CD105
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human CD105.
Database link: P17813
Positive control
- WB: HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC-12, MCF7 and pCMV6-ENTRY CD105-transfected HEK-293T whole cell lysate. IHC-P: Human spleen tissue. ICC/IF: HeLa cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

8A1
Isotype

IgG2b
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [8A1] (ab230925)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD105 using ab230925 at 10 μg/ml in immunohistochemical analysis.
Western blot - Anti-CD105 antibody [8A1] (ab230925)

All lanes : Anti-CD105 antibody [8A1] (ab230925) at 1/200 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SVT2 whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 71 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [8A1] (ab230925)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD105 (green) using ab230925 at 1/100 dilution in ICC/IF.
Western blot - Anti-CD105 antibody [8A1] (ab230925)

All lanes : Anti-CD105 antibody [8A1] (ab230925) at 1/200 dilution

Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY CD105-transfected HEK-293T whole cell lysate

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.

Predicted band size: 71 kDa

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