Anti-Cbx6 antibody [EPR23828-108] (ab259848)
![Anti-Cbx6 antibody [EPR23828-108] (ab259848)](https://www.abcam.com/ps/products/259/ab259848/Images/ab259848-451598-anti-cbx6-antibody-epr23828108-western-blot-kidney.jpg "Anti-Cbx6 antibody [EPR23828-108] (ab259848)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23828-108] to Cbx6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cbx6 antibody [EPR23828-108]
See all Cbx6 primary antibodies -
Description
Rabbit monoclonal [EPR23828-108] to Cbx6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T and NIH/3T3 whole cell lysates; Human colon and testis tissue lysates. IHC-P: Human, mouse and rat cerebrum tissue. Human colon tissue. Human hepatocellular carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23828-108 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cbx6 antibody (ab259848)All lanes : Anti-Cbx6 antibody [EPR23828-108] (ab259848) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : Human colon tissue lysate
Lane 3 : Human testis tissue lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259848)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human cerebrum (PMID: 24260522). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259848)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on the immune cells of human colon. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259848)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human hepatocellular carcinoma (PMID: 28122351). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259848)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259848)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).