Anti-Cathepsin D antibody [CTD-19] (ab6313)
![Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.com/ps/products/6/ab6313/Images/ab6313-275029-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg "Anti-Cathepsin D antibody [CTD-19] (ab6313)")
Key features and details
- Mouse monoclonal [CTD-19] to Cathepsin D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cathepsin D antibody [CTD-19]
See all Cathepsin D primary antibodies -
Description
Mouse monoclonal [CTD-19] to Cathepsin D -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length native protein (purified) corresponding to Human Cathepsin D.
Database link: P07339 -
Positive control
- Human breast carcinoma tissue or cell line extract.
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General notes
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.097% Sodium azide -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
CTD-19 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313) This image is courtesy of an anonymous AbreviewAnti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
Secondary
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.com/ps/products/6/ab6313/Images/ab6313-12229-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg)
Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cellsThis image was generated using the ascites version of the product.
