Anti-AK 1 antibody [EP7083] (ab138504)
![Anti-AK 1 antibody [EP7083] (ab138504)](https://www.abcam.com/ps/products/138/ab138504/Images/ab138504-393632-anti-ak-1-antibody-ep7083-western-blot-human-knockout-lysate.jpg "Anti-AK 1 antibody [EP7083] (ab138504)")
Key features and details
- Rabbit monoclonal [EP7083] to AK 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AK 1 antibody [EP7083]
See all AK 1 primary antibodies -
Description
Rabbit monoclonal [EP7083] to AK 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human AK 1 aa 100-150. The exact sequence is proprietary.
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Positive control
- WB: HeLa, U87-MG, Saos-2, HT-29, fetal muscle, and fetal heart lysates.
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General notes
Previously labelled as Adenylate Kinase 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7083 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AK 1 antibody [EP7083] (ab138504)All lanes : Anti-AK 1 antibody [EP7083] (ab138504) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : AK1 knockout HeLa cell lysate
Lane 3 : U-87MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab138504 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138504 Anti-AK 1 antibody [EP7083] was shown to specifically react with AK 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265132 (knockout cell lysate ab257824) was used. Wild-type and AK 1 knockout samples were subjected to SDS-PAGE. ab138504 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AK 1 antibody [EP7083] (ab138504)](https://www.abcam.com/ps/products/138/ab138504/Images/ab138504-153789-anti-ak-1-antibody-ep7083-western-blot.jpg)
Western blot - Anti-AK 1 antibody [EP7083] (ab138504)All lanes : Anti-AK 1 antibody [EP7083] (ab138504) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : U87-MG lysate
Lane 3 : Saos2 lysate
Lane 4 : HT-29 lysate
Lane 5 : fetal muscle lysate
Lane 6 : fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
