Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)
Key features and details
- Goat polyclonal to Casein Kinase 1 delta/CSNK1D
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Casein Kinase 1 delta/CSNK1D antibody
See all Casein Kinase 1 delta/CSNK1D primary antibodies -
Description
Goat polyclonal to Casein Kinase 1 delta/CSNK1D -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta/CSNK1D.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human Casein Kinase 1 delta/CSNK1D aa 310-324 (internal sequence).
Sequence:DREERLRHSRNPATR
Database link: P48730 -
Positive control
- WB: HEK 293 cell lysate. ICC/UF U251 cells.
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General notes
Previously labelled as Casein Kinase 1 delta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031) at 0.3 µg/ml + HEK 293 cell lysate in RIPA buffer at 35 µg
Predicted band size: 47 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling Casein Kinase 1 delta/CSNK1D with ab48031 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
