Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 delta/CSNK1D
- Suitable for: IP, ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Casein Kinase 1 delta/CSNK1D antibody
See all Casein Kinase 1 delta/CSNK1D primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 1 delta/CSNK1D -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human Casein Kinase 1 delta/CSNK1D aa 150-400.
Database link: P48730 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human skeletal muscle, liver cancer and lymph node tissue. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.
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General notes
Previously labelled as Casein Kinase 1 delta.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)Paraffin-embedded human skeletal muscle tissue stained for Casein Kinase 1 delta/CSNK1D with ab236601 at a 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Casein Kinase 1 delta/CSNK1D using ab236601 at a dilution of 1/333 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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Immunoprecipitation - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)Casein Kinase 1 delta/CSNK1D was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using 6 µg of ab236601.
Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.
Lane 2: ab236601 (6 µg) in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)Paraffin-embedded human liver cancer tissue stained for Casein Kinase 1 delta/CSNK1D with ab236601 at a 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)Paraffin-embedded human lymph node tissue stained for Casein Kinase 1 delta/CSNK1D with ab236601 at a 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
