Anti-Rel B antibody [EP614Y] - BSA and Azide free (ab247279)
![Anti-Rel B antibody [EP614Y] - BSA and Azide free (ab247279)](https://www.abcam.com/ps/products/247/ab247279/Images/ab247279-395229-anti-rel-b-antibody-ep614y-western-blot-hela-raji-lncap-lysates.jpg "Anti-Rel B antibody [EP614Y] - BSA and Azide free (ab247279)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP614Y] to Rel B - BSA and Azide free
- Suitable for: Flow Cyt, WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Rel B antibody [EP614Y] - BSA and Azide free
See all Rel B primary antibodies -
Description
Rabbit monoclonal [EP614Y] to Rel B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247279 is the carrier-free version of ab33907 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247279 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP614Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rel B antibody [EP614Y] - BSA and Azide free (ab247279)All lanes : Anti-Rel B antibody [EP614Y] (ab33907) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RELB knockout HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : LnCap cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab33907).
Lanes 1- 4: Merged signal (red and green). Green - ab33907 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab33907 was shown to react with Rel B in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265948 (knockout cell lysate ab257635) was used. Wild-type HeLa and RELB knockout HeLa cell lysates were subjected to SDS-PAGE. ab33907 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Rel B antibody [EP614Y] - BSA and Azide free (ab247279)](https://www.abcam.com/ps/products/247/ab247279/Images/ab247279-2-benefits-of-recombinant-antibodies.png)
Anti-Rel B antibody [EP614Y] - BSA and Azide free (ab247279)
