Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit Monoclonal [EPCEAR7] to CEACAM5
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-CEACAM5 antibody [EPCEAR7]
See all CEACAM5 primary antibodies
Description

Rabbit Monoclonal [EPCEAR7] to CEACAM5
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Full length native protein (purified) corresponding to Human Carcino Embryonic Antigen CEA.
Positive control
- Human colon cancer lysate, Human colon carcinoma tissue, MCF-7 and human gastric carcinoma.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPCEAR7
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Carcino Embryonic Antigen CEA with Purified ab133633 at 1:3000 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633) at 1/1000 dilution + Human colon cancer lysate at 10 µg

Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 76 kDa
Observed band size: 180-200 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Confocal image showing membranous staining in MCF7 cell line.

Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labelling carcino embryonic antigen (CEA) with ab133633 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/10000 dilution was used as the secondary antibody. The cells were counterstained with ab195889, a anti-alpha tubulin antibody at 1/200 dilution. DAPI was used as a nuclear counterstain.

Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling Carcino Embryonic Antigen CEA with Purified ab133633 at 1:3000 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing positive staining in Normal colon tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labelling Carcino Embryonic Antigen CEA with ab133633 at 1/250.
Western blot - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633) at 0.4 µg/ml (Purified) + Human colon cancer lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 76 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing positive staining in Stomach adenocarcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing negative staining in Skeletal muscle tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing negative staining in Normal tonsil tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing negative staining in Thyroid gland carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing negative staining in Hepatocellular carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

ab133633 showing negative staining in Glioma tissue.
Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (ab133633)

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