Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] - BSA and Azide free (ab248723)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] - BSA and Azide free
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human adrenal gland lysate, mouse and rat testis lysate. IHC-P: Human and mouse testis tissues.
-
General notes
ab248723 is the carrier-free version of ab134910 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248723 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Cytochrome P450 17A1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6294(2) -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse Leydig cells.The section was incubated with ab134910 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate,
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
-
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/10000 dilution + Human adrenal gland lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 57 kDaThis data was developed using ab134910, the same antibody clone in a different buffer formulation.
-
This data was developed using ab134910, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-