Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [005] to Carbonic Anhydrase 8/CA8
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Carbonic Anhydrase 8/CA8 antibody [005]
    See all Carbonic Anhydrase 8/CA8 primary antibodies

  • Description

    Rabbit monoclonal [005] to Carbonic Anhydrase 8/CA8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Mouse Carbonic Anhydrase 8/CA8 aa 1 to the C-terminus.
    Database link: P28651

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T and A549 whole cell lysate. IP: HEK-293T and A549 whole cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    005

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
    Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
    All lanes : Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Performed under reducing conditions.


    Developed using the Odyssey technique. 

  • Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
    Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)

    Carbonic Anhydrase 8 was immunoprecipitated from 0.5 mg of HEK-293T whole cell lysate (Lane A) or A549 whole cell lysate (Lane B) with 2 µL of ab275656 and 15 µL of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab275656 at 1/200 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
    Developed using the Odssey technique.

  • Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
    Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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