Anti-AS160 (phospho S588) antibody [5HCLC] (ab277781)
![Anti-AS160 (phospho S588) antibody [5HCLC] (ab277781)](https://www.abcam.com/ps/products/277/ab277781/Images/ab277781-2-antiPhosphoAS160S588recombinantpolyclonalantibody5hclcWB.jpg "Anti-AS160 (phospho S588) antibody [5HCLC] (ab277781)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [5HCLC] to AS160 (phospho S588)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-AS160 (phospho S588) antibody [5HCLC]
See all AS160 primary antibodies -
Description
Rabbit recombinant multiclonal [5HCLC] to AS160 (phospho S588) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 583-592 (phospho S588).
Database link: O60343 -
General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
5HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AS160 antibody [5HCLC] (ab277781)All lanes : Anti-AS160 (phospho S588) antibody [5HCLC] (ab277781) at 2.5 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract (treated with insulin, 30 nmol / L for 15 minutes)
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract (treated with IGF1, 100 ng / mL for 15 minutes)
Developed using the ECL technique.
Predicted band size: 146 kDa
Exposure time: 15 minutes
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Immunocytochemistry - Anti-AS160 antibody (ab277781)For immunofluorescence analysis HT-29 cells were fixed and permeabilized for detection of endogenous AS160[pS588] using Anti-AS160[pS588] Recombinant Rabbit Multiclonal Antibody (ab277781, 5 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor®® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI, and Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of AS160[Ps588] (green) in the cytoplasm can be clearly observed in cells treated with IGF (100 ng/mL, 15 min) as compared to untreated cells. Antibody specificity was demonstrated by competition with the AS160[Ps588] phospho peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.
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![Anti-AS160 antibody [5HCLC] (ab277781)](https://www.abcam.com/ps/products/277/ab277781/Images/ab277781-3-benefits-of-recombinant-antibodies.jpg)
Anti-AS160 antibody [5HCLC] (ab277781)