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Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

Price and availability

291 484 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [22B1] to CaMKII (phospho T286)
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CaMKII (phospho T286) antibody [22B1]
    See all CaMKII primary antibodies
  • Description

    Mouse monoclonal [22B1] to CaMKII (phospho T286)
  • Host species

    Mouse
  • Specificity

    ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Rat
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Rat CaMKII aa 281-294 (phospho T286).
    Sequence:

    HRQETVDCLKKFN


    Database link: P11275
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Neuro-2a, C6, HeLa or U251 cells, Rat brain lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    22B1
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
    Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
    Lanes 1-2 : Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) at 1/2000 dilution
    Lanes 3-4 : A non phosphorylated CaMKII antibody at 1/2000 dilution

    Lane 1 : Unphosphorylated rat brain homogenate
    Lanes 2 & 4 : Phosphorylated Rat brain homogenate
    Lane 3 : Unphosphorylated Rat brain homogenate

    Predicted band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of U251 (human) cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of Neuro-2 (mouse) cells (left image) or cells stimulated with Lambda-phosphatase (right image) labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. F-actin (red) and nuclei (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of HeLa cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution.  Nuclei are stained (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of C6 cells labeling phospohrylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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