Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
Key features and details
- Mouse monoclonal [22B1] to CaMKII (phospho T286)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CaMKII (phospho T286) antibody [22B1]
See all CaMKII primary antibodies -
Description
Mouse monoclonal [22B1] to CaMKII (phospho T286) -
Host species
Mouse -
Specificity
ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseRatHumanWB Rat -
Immunogen
Synthetic peptide corresponding to Rat CaMKII aa 281-294 (phospho T286).
Sequence:HRQETVDCLKKFN
Database link: P11275 -
Positive control
- Neuro-2a, C6, HeLa or U251 cells, Rat brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
22B1 -
Isotype
IgG1 -
Research areas
Images
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Lanes 1-2 : Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) at 1/2000 dilution
Lanes 3-4 : A non phosphorylated CaMKII antibody at 1/2000 dilution
Lane 1 : Unphosphorylated rat brain homogenate
Lanes 2 & 4 : Phosphorylated Rat brain homogenate
Lane 3 : Unphosphorylated Rat brain homogenate
Predicted band size: 54 kDa
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Immunofluorescence analysis of U251 (human) cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).
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Immunofluorescence analysis of Neuro-2 (mouse) cells (left image) or cells stimulated with Lambda-phosphatase (right image) labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. F-actin (red) and nuclei (blue).
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Immunofluorescence analysis of HeLa cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. Nuclei are stained (blue).
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Immunofluorescence analysis of C6 cells labeling phospohrylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).