Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2562Y] to Calpain 2 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free
    See all Calpain 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2562Y] to Calpain 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, A431 and HeLa cell lysates. Flow Cyt: HeLa cells.

  • General notes

    ab247423 is the carrier-free version of ab75994 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247423 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    Western blot - Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    All lanes : Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/2000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate at 40 µg
    Lane 2 : CAPN2 knockout HEK-293T cell lysate at 40 µg
    Lane 3 : A431 cell lysate at 20 µg
    Lane 4 : LNCaP cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab75994).

    Lanes 1 - 4: Merged signal (red and green). Green - ab75994 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab75994 was shown to react with Calpain 2 in wild-type HEK-293T cells in Western blot with loss of signal observed in CAPN2 knockout cell line ab266628 (CAPN2 knockout cell lysate ab257379). Wild-type HEK-293T and CAPN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab75994 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    Flow Cytometry - Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    This data was developed using ab75994, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells using ab75994 (red) or a rabbit IgG (negative) (green).
  • Western blot - Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    Western blot - Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 80 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    This data was developed using ab75994, the same antibody clone in a different buffer formulation.

  • Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)
    Anti-Calpain 2 antibody [EPR2562Y] - BSA and Azide free (ab247423)

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