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Anti-Calcitonin antibody [16B5] (ab11493)

Key features and details

  • Mouse monoclonal [16B5] to Calcitonin
  • Suitable for: Sandwich ELISA, Flow Cyt, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Calcitonin antibody [16B5]
    See all Calcitonin primary antibodies

  • Description

    Mouse monoclonal [16B5] to Calcitonin

  • Host species

    Mouse

  • Specificity

    This antibody recognises human procalcitonin and calcitonin. Specific for calcitonin (central part).

  • Tested applications

    Suitable for: Sandwich ELISA, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment (Human).

  • Epitope

    aa 72-81

  • General notes

    This product was changed from ascites to tissue culture supernatant on 13 June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)
    Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)

    Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5 ] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml

    This image was generated using the ascites version of the product.

  • Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)
    Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)

    Calibration curves of several procalcitonin sandwich ELISAs using a range of monclonal antibodies available agaisn't procalcitonin, calcitonin and catacalcin fragments. Capture antibodies used at 1 µg/well and detection antibodies at 0.1 µg/well.

    Dark blue square = ab11493 [16B5] (calcitonin fragment) and ab11494 [42] (procalcitonin fragment). Light grey circle = ab11497 [24B2] (calcitonin fragment) and ab11484 [13B9] (calcitonin fragment). Grey triangle = ab11487 [14C12] (catacalcin fragment) and ab11496 [14A2] (calcitonin fragment). Dark grey circle = ab14813 [27A3] (procalcitonin fragment) and ab11491 [22A11] (catacalcin fragment).

    This image was generated using the ascites version of the product.

  • Schematic Diagram - Anti-Calcitonin antibody [16B5] (ab11493)
    Schematic Diagram - Anti-Calcitonin antibody [16B5] (ab11493)

    Amino acid sequence and schematic diagram of human procalcitonin and the N-terminal, calcitonin, and katacalcin (catacalcin) fragments.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Calcitonin antibody [16B5] (ab11493)
    Western blot - Anti-Calcitonin antibody [16B5] (ab11493)

    Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

    Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab11498 [38F11], 4) ab14817 [44D9], 5) ab11494 [42],

    Anti- calcitonin antibodies available; 6) ab11484 [13B9], 7) ab14818 [13F2], 8) ab14819 [13G11], 9) ab11496 [14A2], 10) ab11493 [16B5], 11) ab11497 [24B2],

    Anti- catacalcin antibodies available; 12) ab11487 [14C12], 13) ab11490 [18B7], 14) ab11491 [22A11].

    Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

    Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab1149

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Calcitonin antibody [16B5] (ab11493)
    Flow Cytometry - Anti-Calcitonin antibody [16B5] (ab11493)

    Overlay histogram showing SH-SY5Y cells stained with ab11493 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11493, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

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