Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calbindin antibody [EPR22698-236] (ab229915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22698-236] to Calbindin
  • Suitable for: IHC-Fr, IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Calbindin antibody [EPR22698-236]
    See all Calbindin primary antibodies

  • Description

    Rabbit monoclonal [EPR22698-236] to Calbindin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and cerebellum lysates; rat brain lysate. IP: Rat brain lysate; mouse brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue. IHC-Fr: Rat cerebellum tissue; mouse cerebellum tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Calbindin with ab229915 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on molecular layer and purkinje cells of mouse cerebellum (PMID: 20130198) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Calbindin with ab229915 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on molecular layer and purkinje cells of rat cerebellum (PMID: 20130198) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Calbindin with ab229915 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Purkinje cells of rat cerebellum (PMID: 17507571) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab229915 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Calbindin with ab229915 at 1/4000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong positive staining on Purkinje cells of mouse cerebellum (PMID: 17507571) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab229915 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] (ab229915)

    Calbindin was immunoprecipitated from 0.35 mg of mouse brain lysate with ab229915 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229915 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain lysate 10 μg (Input).
    Lane 2: ab229915 IP in mouse brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229915 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

  • Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] (ab229915)

    Calbindin was immunoprecipitated from 0.35 mg of rat brain lysate with ab229915 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229915 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Rat brain lysate 10 μg (Input). 
    Lane 2: ab229915 IP in rat brain lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229915 in rat brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Western blot - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Western blot - Anti-Calbindin antibody [EPR22698-236] (ab229915)
    All lanes : Anti-Calbindin antibody [EPR22698-236] (ab229915) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : C6 (rat glial tumor glial cell line) whole cell lysate
    Lane 5 : Mouse cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 15 seconds; Lanes 3 & 4: 3 minutes; Lane 5: 48 seconds.

    Negative control: NIH/3T3, C6 (lanes 2 & 4, PMID: 8370070, 9889325).

  • Anti-Calbindin antibody [EPR22698-236] (ab229915)
    Anti-Calbindin antibody [EPR22698-236] (ab229915)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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