Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Calbindin antibody [EPR22698-236] (ab229915)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22698-236] to Calbindin
Suitable for: IHC-Fr, IHC-P, WB, IP
Reacts with: Mouse, Rat

Product name

Anti-Calbindin antibody [EPR22698-236]
See all Calbindin primary antibodies
Description

Rabbit monoclonal [EPR22698-236] to Calbindin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse Rat
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain and cerebellum lysates; rat brain lysate. IP: Rat brain lysate; mouse brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue. IHC-Fr: Rat cerebellum tissue; mouse cerebellum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22698-236
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Calbindin with ab229915 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on molecular layer and purkinje cells of mouse cerebellum (PMID: 20130198) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Calbindin with ab229915 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on molecular layer and purkinje cells of rat cerebellum (PMID: 20130198) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Calbindin with ab229915 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Purkinje cells of rat cerebellum (PMID: 17507571) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab229915 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] (ab229915)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Calbindin with ab229915 at 1/4000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong positive staining on Purkinje cells of mouse cerebellum (PMID: 17507571) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab229915 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] (ab229915)

Calbindin was immunoprecipitated from 0.35 mg of mouse brain lysate with ab229915 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229915 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

Lane 1: Mouse brain lysate 10 μg (Input).
Lane 2: ab229915 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229915 in mouse brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] (ab229915)

Calbindin was immunoprecipitated from 0.35 mg of rat brain lysate with ab229915 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229915 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

Lane 1: Rat brain lysate 10 μg (Input).
Lane 2: ab229915 IP in rat brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229915 in rat brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Western blot - Anti-Calbindin antibody [EPR22698-236] (ab229915)

All lanes : Anti-Calbindin antibody [EPR22698-236] (ab229915) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : Rat brain lysate
Lane 4 : C6 (rat glial tumor glial cell line) whole cell lysate
Lane 5 : Mouse cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1 & 2: 15 seconds; Lanes 3 & 4: 3 minutes; Lane 5: 48 seconds.

Negative control: NIH/3T3, C6 (lanes 2 & 4, PMID: 8370070, 9889325).
Anti-Calbindin antibody [EPR22698-236] (ab229915)

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