Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free
See all Calbindin primary antibodies
Description

Rabbit monoclonal [EPR22698-236] to Calbindin - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IHC-Fr, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IP: Rat brain lysate; mouse brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue. IHC-Fr: Rat cerebellum tissue; mouse cerebellum tissue.
General notes
ab255691 is the carrier-free version of ab229915.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22698-236
Isotype

IgG
Research areas
- Neuroscience
- Processes

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Calbindin with ab229915 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on molecular layer and purkinje cells of mouse cerebellum (PMID: 20130198) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229915).
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Calbindin with ab229915 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on molecular layer and purkinje cells of rat cerebellum (PMID: 20130198) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229915).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Calbindin with ab229915 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Purkinje cells of rat cerebellum (PMID: 17507571) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab229915 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229915).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Calbindin with ab229915 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong positive staining on Purkinje cells of mouse cerebellum (PMID: 17507571) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab229915 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229915).
Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)

Calbindin was immunoprecipitated from 0.35 mg of mouse brain lysate with ab229915 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229915 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

Lane 1: Mouse brain lysate 10 μg (Input).
Lane 2: ab229915 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229915 in mouse brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229915).
Immunoprecipitation - Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)

Calbindin was immunoprecipitated from 0.35 mg of rat brain lysate with ab229915 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229915 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

Lane 1: Rat brain lysate 10 μg (Input).
Lane 2: ab229915 IP in rat brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229915 in rat brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229915).
Anti-Calbindin antibody [EPR22698-236] - BSA and Azide free (ab255691)