Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C4a antibody [EPR10143] (ab170942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10143] to C4a
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-C4a antibody [EPR10143]
    See all C4a primary antibodies

  • Description

    Rabbit monoclonal [EPR10143] to C4a

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 97% homology with C4b. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human C4a aa 900-1250. The exact sequence is proprietary.
    Database link: P0C0L4

  • Positive control

    • HepG2 cell lysate; Human colon and stomach tissues; HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10143

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C4a antibody [EPR10143] (ab170942)
    Western blot - Anti-C4a antibody [EPR10143] (ab170942)
    Anti-C4a antibody [EPR10143] (ab170942) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Predicted band size: 193 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

    ab170942 showing +ve staining in Human normal lung tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

    ab170942 showing +ve staining in Human normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

    ab170942 showing +ve staining in Human thyroid gland carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling C4a with ab170942 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling C4a with ab170942 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-C4a antibody [EPR10143] (ab170942)
    Flow Cytometry - Anti-C4a antibody [EPR10143] (ab170942)

    Flow cytometric analysis of permeabilized HepG2 cells labeling C4a using ab170942 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

  • Anti-C4a antibody [EPR10143] (ab170942)
    Anti-C4a antibody [EPR10143] (ab170942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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