Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10143] to C4a
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-C4a antibody [EPR10143]
See all C4a primary antibodies
Description

Rabbit monoclonal [EPR10143] to C4a
Host species

Rabbit
Specificity

The immunogen used for this product shares 97% homology with C4b. Cross-reactivity with this protein has not been confirmed experimentally.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human C4a aa 900-1250. The exact sequence is proprietary.
Database link: P0C0L4
Positive control
- HepG2 cell lysate; Human colon and stomach tissues; HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10143
Isotype

IgG
Research areas

Western blot - Anti-C4a antibody [EPR10143] (ab170942)

Anti-C4a antibody [EPR10143] (ab170942) at 1/1000 dilution + HepG2 cell lysate at 10 µg

Predicted band size: 193 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

ab170942 showing +ve staining in Human normal lung tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

ab170942 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

ab170942 showing +ve staining in Human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling C4a with ab170942 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4a antibody [EPR10143] (ab170942)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling C4a with ab170942 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-C4a antibody [EPR10143] (ab170942)

Flow cytometric analysis of permeabilized HepG2 cells labeling C4a using ab170942 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
Anti-C4a antibody [EPR10143] (ab170942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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