Anti-Alpha-synuclein (phospho Y136) antibody (ab131491)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho Y136)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Alpha-synuclein (phospho Y136) antibody
See all Alpha-synuclein primary antibodies -
Description
Rabbit polyclonal to Alpha-synuclein (phospho Y136) -
Host species
Rabbit -
Specificity
ab131491 detects endogenous levels of alpha Synuclein only when phosphorylated at tyrosine 136. Due to 72.7% sequence homology ab131491 might react with Beta synuclein -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Alpha-synuclein aa 134-138 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH, surrounding phosphorylation site of Tyrosine 136 (Q-D-Y(p)-E-P) derived from Human alpha synuclein (NP_000336.1)
Sequence:QDYEP
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Positive control
- Mouse brain tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131491 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y136) antibody (ab131491)Immunofluorescence analysis of methanol-fixed HeLa cells labelling alpha Synuclein using ab131491, at 1/100 dilution.
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Western blot - Anti-Alpha-synuclein (phospho Y136) antibody (ab131491)All lanes : Anti-Alpha-synuclein (phospho Y136) antibody (ab131491) at 1/500 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse brain tissue lysate with blocking peptide
Predicted band size: 14 kDa
