Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB
  • Suitable for: IHC-P, WB, IP, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-C4 binding protein/C4BPB antibody [EPR17101]
    See all C4 binding protein/C4BPB primary antibodies

  • Description

    Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma and human ovary cancer cell extracts. Human serum. Rat plasma. IHC-P: Human hepatocellular carcinoma and human bladder carcinoma tissue. Rat liver tissue. ICC/IF: HepG2 cells. IP: Human serum.

  • General notes

     

     

     This product was previously labelled as C4 binding protein

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17101

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    All lanes : Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human ovary cancer extract.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

     

    The observed MW is consistent with what has been described in the literature (PMID: 2300577)

  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/10000 dilution + Human serum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 2300577)

  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution + Rat plasma at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 2300577)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

    Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Plasma staining on Human bladder carcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Immunocytochemistry/ Immunofluorescence - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling C4 binding protein/C4BPB with ab199430 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab199430 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

    C4 binding protein/C4BPB was immunoprecipitated from 1mg of Human serum with ab199430 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab199430 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human serum 10 µg. Lane 2: Human serum following immunoprecipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199430 in Human serum.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure: 10 seconds.

  • Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
    Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

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