Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10495] to C11B2/CYP11B2
Suitable for: WB, IHC-P, IP, Flow Cyt
Reacts with: Human

Product name

Anti-C11B2/CYP11B2 antibody [EPR10495]
See all C11B2/CYP11B2 primary antibodies
Description

Rabbit monoclonal [EPR10495] to C11B2/CYP11B2
Host species

Rabbit
Specificity

In house testing has shown no cross-reactivity with C11B1 in either western blot (see image) or peptide ELISA.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human C11B2/CYP11B2 aa 300-400. The exact sequence is proprietary.
Database link: P19099
Positive control
- Human adrenal gland, NIH:OVCAR-3 and MCF7 cell lysates; Human heart and Human kidney tissues.
General notes
This product was previously labelled as C11B2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10495
Isotype

IgG
Research areas

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

Lane 1 : MCF-7 cell lysate with Human C11B2/CYP11B2 peptide
Lane 2 : MCF-7 cell lysate with Human C11B1/CYP11B2 peptide
Lane 3 : MCF-7 cell lysate with no blocking peptide

Predicted band size: 58 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds
Flow Cytometry - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Overlay histogram showing MCF7 cells stained with ab167413 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab167413, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

Lane 1 : Human adrenal gland tissue lysate
Lane 2 : NIH:OVCAR-3 cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C11B2/CYP11B2 with ab167413 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C11B2/CYP11B2 with ab167413 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Detection of C11B2/CYP11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.
Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

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