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Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10495] to C11B2/CYP11B2
  • Suitable for: WB, IHC-P, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-C11B2/CYP11B2 antibody [EPR10495]
    See all C11B2/CYP11B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10495] to C11B2/CYP11B2

  • Host species

    Rabbit

  • Specificity

    In house testing has shown no cross-reactivity with C11B1 in either western blot (see image) or peptide ELISA.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human C11B2/CYP11B2 aa 300-400. The exact sequence is proprietary.
    Database link: P19099

  • Positive control

    • Human adrenal gland, NIH:OVCAR-3 and MCF7 cell lysates; Human heart and Human kidney tissues.

  • General notes

     

     

     This product was previously labelled as C11B2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate with Human C11B2/CYP11B2 peptide
    Lane 2 : MCF-7 cell lysate with Human C11B1/CYP11B2 peptide
    Lane 3 : MCF-7 cell lysate with no blocking peptide

    Predicted band size: 58 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds
  • Flow Cytometry - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Flow Cytometry - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Overlay histogram showing MCF7 cells stained with ab167413 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab167413, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

    Lane 1 : Human adrenal gland tissue lysate
    Lane 2 : NIH:OVCAR-3 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C11B2/CYP11B2 with ab167413 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C11B2/CYP11B2 with ab167413 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

    Detection of C11B2/CYP11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.

  • Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
    Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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