Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23975-119] to C Reactive Protein - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free
    See all C Reactive Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR23975-119] to C Reactive Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver, and kidney tissue lysates: Human plasma; Mouse plasma and serum; Rat liver and kidney tissue lysates. IHC-P: Mouse liver tissue: Rat liver tissue. IP: Mouse serum.

  • General notes

    ab280362 is the carrier-free version of ab259862. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280362 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)

    This data was developed using ab259862, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling C Reactive Protein with ab259862 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse liver. The section was incubated with ab259862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    All lanes : Anti-C Reactive Protein antibody [EPR23975-119] (ab259862) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human kidney tissue lysate
    Lane 3 : Human plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259862, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 

    Exposure time: 3.25 seconds

  • Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)

    This data was developed using ab259862, the same antibody clone in a different buffer formulation.

    C Reactive Protein was immunoprecipitated from 0.35 mg Mouse serum with ab259862 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259862 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse serum 10 ug

    Lane 2: ab259862 IP in Mouse serum

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259862 in mouse serum

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)

    This data was developed using ab259862, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C Reactive Protein with ab259862 at 1/10000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat liver. The section was incubated with ab259862 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    All lanes : Anti-C Reactive Protein antibody [EPR23975-119] (ab259862) at 1/2000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    This data was developed using ab259862, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 37 seconds

  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    All lanes : Anti-C Reactive Protein antibody [EPR23975-119] (ab259862) at 1/1000 dilution

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    This data was developed using ab259862, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: 1 second

  • Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)
    Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (ab280362)

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