Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP769Y] to c-Myb (phospho S11) - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free
    See all c-Myb primary antibodies

  • Description

    Rabbit monoclonal [EP769Y] to c-Myb (phospho S11) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Dot blotmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human cervical carcinoma tissue.

  • General notes

    ab217945 is the carrier-free version of ab45150 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab217945 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)
    Dot Blot - Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)

    Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labeling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)

    Unpurified ab45150 staining human v-Myb / c-Myb in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)
    Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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