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Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

Key features and details

  • Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-v-Myb + c-Myb (phospho S12) antibody

  • Description

    Rabbit polyclonal to v-Myb + c-Myb (phospho S12)

  • Host species

    Rabbit

  • Specificity

    ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

  • Positive control

    • Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
    Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
  • Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
    Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
    All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated
    with Hu (2nM, 24hours)
    Lane 2 : Extracts from HeLa cells treated
    with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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