All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 35 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20976196).

Negative control: T-47D (PMID: 24830720).

Exposure time: lane 1: 3 minutes
lane 2, 3:15 seconds
lane 4: 37 seconds

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human esophagus.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling c-Jun with ab280089 at 1/50 (19.56 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in MDA-MB-231 cells.

Negative control: MCF7 (PMID: 24830720).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab280089 at a 1/50 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 35 kDa

Lysates were made freshly and used in WB immediately to minimize protein degradation.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: lane 1: 3 mins;

lane 2, 3: 15 secs;

lane 4: 37 secs.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Scattered nuclear staining on human colon.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of mouse hippocampus.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse cerebrum.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of rat hippocampus.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).