Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µg 351 792 ₸ 10 µg 117 264 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [3/Jun] to c-Jun
Suitable for: IHC-P, ICC, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-c-Jun antibody [3/Jun]
See all c-Jun primary antibodies
Description

Mouse monoclonal [3/Jun] to c-Jun
Host species

Mouse

Tested Applications & Species

Application	Species
ICC	Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, HeLa, NIH/3T3. PC-12 and MDA-MB-231 whole cell lysate. IHC-P: Human esophagus and colon tissue. Mouse and rat hippocampus tissue. Mouse and rat cerebrum tissue. ICC: MDA-MB-231 cells.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

3/Jun
Isotype

IgG2a
Research areas

Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)

All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 35 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20976196).

Negative control: T-47D (PMID: 24830720).

Exposure time: lane 1: 3 minutes
lane 2, 3:15 seconds
lane 4: 37 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human esophagus.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunocytochemistry - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling c-Jun with ab280089 at 1/50 (19.56 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in MDA-MB-231 cells.

Negative control: MCF7 (PMID: 24830720).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab280089 at a 1/50 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)

All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 35 kDa

Lysates were made freshly and used in WB immediately to minimize protein degradation.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: lane 1: 3 mins;

lane 2, 3: 15 secs;

lane 4: 37 secs.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Scattered nuclear staining on human colon.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of mouse hippocampus.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse cerebrum.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of rat hippocampus.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum.

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Anti-c-Jun antibody [3/Jun] (ab280089)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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