Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bub1 antibody [EPR18947] - BSA and Azide free (ab251203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18947] to Bub1 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bub1 antibody [EPR18947] - BSA and Azide free
    See all Bub1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18947] to Bub1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody shows stronger signal in mouse and rat testis tissues but weaker in the human testis.

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251203 is the carrier-free version of ab195268 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251203 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our in house IHC testing showed positive staining in testis tissue ONLY. Other tissues were negative.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Bub1 antibody [EPR18947] - BSA and Azide free (ab251203)
    Western blot - Anti-Bub1 antibody [EPR18947] - BSA and Azide free (ab251203)
    All lanes : Anti-Bub1 antibody [EPR18947] (ab195268) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : BUB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab195268).

    Lanes 1- 2: Merged signal (red and green). Green - ab195268 observed at 125 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab195268 was shown to react with Bub1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265145 (knockout cell lysate ab257373) was used. Wild-type HeLa and BUB1 knockout HeLa cell lysates were subjected to SDS-PAGE. ab195268 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Bub1 antibody [EPR18947] - BSA and Azide free (ab251203)
    Anti-Bub1 antibody [EPR18947] - BSA and Azide free (ab251203)

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