Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BtkY223-B4] to BTK (phospho Y223)
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BTK (phospho Y223) antibody [BtkY223-B4]
    See all BTK primary antibodies

  • Description

    Rabbit monoclonal [BtkY223-B4] to BTK (phospho Y223)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BTK (phospho Y223). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q06187

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow cyt: U937 and NIH/3T3 cells (both treated with IFNa, IL4 and PV)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    BtkY223-B4

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)
    Flow Cytometry - Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)

    Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa IL4 and PV (green) using 5 ng/mL of ab278680.

  • Flow Cytometry - Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)
    Flow Cytometry - Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)
    Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or IFNa + IL4 + pervanadate-treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) ab278680 at 0.025µg/mL.
  • Flow Cytometry - Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)
    Flow Cytometry - Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)

    Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with IFNa + IL4 + pervanadate (orange) using 0.05 µg/mL isotype control, or untreated (red) or treated (green) using ab278680 at 0.05µg/mL.

  • Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)
    Anti-BTK (phospho Y223) antibody [BtkY223-B4] (ab278680)

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