Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRSK1 antibody [EPR18190] (ab206298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18190] to BRSK1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRSK1 antibody [EPR18190]
    See all BRSK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18190] to BRSK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y, Jurkat, PC-12 and NIH/3T3 whole cell lysates; mouse testis lysate; rat brain lysate. ICC/IF: Neuro-2a and NIH/3T3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18190

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298)
    Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298)
    All lanes : Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : Mouse testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298)
    Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298)
    All lanes : Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298)
    Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298)
    All lanes : Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    Exposure time: Lane 1 - 30 seconds; Lane 2 - 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (ab206298)
    Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (ab206298)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling BRSK1 with ab206298 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab206298 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution

  • Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (ab206298)
    Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (ab206298)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling BRSK1 with ab206298 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab206298 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution

  • Anti-BRSK1 antibody [EPR18190] (ab206298)
    Anti-BRSK1 antibody [EPR18190] (ab206298)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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