Anti-Borrelia burgdorferi IgG ELISA Kit (ab178635)
Key features and details
- Sensitivity: 98.6 %
- Sample type: Cit plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Indirect
Overview
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Product name
Anti-Borrelia burgdorferi IgG ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos serum 7 4.6% Inter-assay Sample n Mean SD CV% Pos serum 3 4% -
Sample type
Serum, Other biological fluids, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
98.6 % -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s anti- Borrelia burgdorferi IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF.
A 96-well plate has been precoated with Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Borrelia burgdorferi (IgG) Coated Microplate (12 x 8 wells) 1 unit Borrelia burgdorferi Anti-IgG HRP Conjugate 1 x 20ml Borrelia burgdorferi Cut-off Control 1 x 3ml Borrelia burgdorferi Negative Control 1 x 2ml Borrelia burgdorferi Positive Control 1 x 2ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
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Relevance
Borrelia burgdorferi is a bacterial species of the spirochete class of the genus Borrelia, which has a diderm (double-membrane) envelope. B. burgdorferi is predominant in North America, but also exists in Europe, and is the agent of Lyme disease. Borrelia burgdorferi is gram negative only by default because safranin is the last dye used, but in reality spirochetes are not classified as either gram positive or negative.
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
