Human Anti-Borrelia burgdorferi IgM ELISA Kit (ab108711)
Key features and details
- Sample type: Cerebral Spinal Fluid, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Borrelia burgdorferi IgM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos. Serum 8 8.6% Inter-assay Sample n Mean SD CV% Pos. Serum 14 4.1% -
Sample type
Serum, Hep Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).
A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Borrelia burgdorferi (IgM) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Borrelia burgdorferi anti-IgM HRP Conjugate Red, Black cap 1 x 20ml Borrelia burgdorferi IgM Cut-off Control Yellow, Green cap 1 x 3ml Borrelia burgdorferi IgM Negative Control Yellow, Blue cap 1 x 2ml Borrelia burgdorferi IgM Positive Control Yellow, Red cap 1 x 2ml Cover foil 1 unit IgM Sample Diluent Green, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
