Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19826] to BNIP3L/NIX (phospho S81) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free
    See all BNIP3L/NIX primary antibodies

  • Description

    Rabbit monoclonal [EPR19826] to BNIP3L/NIX (phospho S81) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251494 is the carrier-free version of ab208190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251494 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BNIP3L

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Dot Blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)
    Dot Blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)
    This data was developed using ab208190, the same antibody clone in a different buffer formulation.Dot blot analysis of BNIP3L/NIX (phospho S81) labeled with ab208190 at 1/1000 dilution. Lane 1: BNIP3L/NIX (phospho S81) peptide; Lane 2: BNIP3L/NIX non-phospho peptide; Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)
    Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)
    All lanes : Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (ab208190) at 1/5000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate
    Lane 2 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, whole cell lysate
    Lane 3 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, lysate then treated with alkaline phosphatase for 1 hour, whole cell lysate
    Lane 4 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX S81A mutant expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 24 kDa
    Observed band size: 40,80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab208190, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10467396).

    The plasmids were kindly provided by our collaborator Dr. Zhong Chen, Zhejiang University.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)
    Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (ab251494)

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