Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free (ab276041)
![Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free (ab276041)](https://www.abcam.com/ps/products/276/ab276041/Images/ab276041-2-AntiBMP2-antibody-7D10G8E2-wb-Caco2-THP1-HeLa-cells.jpg "Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free (ab276041)")
Key features and details
- Mouse monoclonal [7D10G8E2] to BMP2 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free
See all BMP2 primary antibodies -
Description
Mouse monoclonal [7D10G8E2] to BMP2 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human BMP2 aa 283-396. The mature form of BMP2 (NP_001191.1). The mature form sequences of human, mouse, rat, rhesus and canine BMP2 are identical.
Database link: P12643 -
Positive control
- WB: THP1, HeLa and Caco-2 whole cell lysates. IP: HeLa, and Caco-2 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7D10G8E2 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-BMP2 antibody [7D10G8E2] (ab276041)All lanes : Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free (ab276041) at 1/500 dilution
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : THP-1 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (Dylight800) at 1/5000 dilution
Predicted band size: 44 kDaPerformed under reducing conditions.
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![Immunoprecipitation - Anti-BMP2 antibody [7D10G8E2] (ab276041)](https://www.abcam.com/ps/products/276/ab276041/Images/ab276041-1-AntiBMP2-antibody-7D10G8E2-IP-HeLa-cells.jpg)
Immunoprecipitation - Anti-BMP2 antibody [7D10G8E2] (ab276041)BMP2 was immunoprecipitated from 0.5 mg HeLa (human colorectal adenocarcinoma cell line) whole cell lysate or 0.5 mg Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate.
0.5 µL ab276041 and 15 μl of 50 % Protein G agarose.
Primary antibody: ab276041, at 1/500 dilution
Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1/7500 dilutionPerformed under reducing conditions.
