Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free (ab276041)

Key features and details

  • Mouse monoclonal [7D10G8E2] to BMP2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free
    See all BMP2 primary antibodies

  • Description

    Mouse monoclonal [7D10G8E2] to BMP2 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human BMP2 aa 283-396. The mature form of BMP2 (NP_001191.1). The mature form sequences of human, mouse, rat, rhesus and canine BMP2 are identical.
    Database link: P12643

  • Positive control

    • WB: THP1, HeLa and Caco-2 whole cell lysates. IP: HeLa, and Caco-2 whole cell lysates.

Properties

Images

  • Western blot - Anti-BMP2 antibody [7D10G8E2] (ab276041)
    Western blot - Anti-BMP2 antibody [7D10G8E2] (ab276041)
    All lanes : Anti-BMP2 antibody [7D10G8E2] - BSA and Azide free (ab276041) at 1/500 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 2 : THP-1 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (Dylight800) at 1/5000 dilution

    Predicted band size: 44 kDa



    Performed under reducing conditions.

  • Immunoprecipitation - Anti-BMP2 antibody [7D10G8E2] (ab276041)
    Immunoprecipitation - Anti-BMP2 antibody [7D10G8E2] (ab276041)

    BMP2 was immunoprecipitated from 0.5 mg HeLa (human colorectal adenocarcinoma cell line) whole cell lysate or 0.5 mg Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate.


    0.5 µL ab276041 and 15 μl of 50 % Protein G agarose.
    Primary antibody: ab276041, at 1/500 dilution
    Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1/7500 dilution

    Performed under reducing conditions.

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