Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [6E9E6] to Properdin/PFC - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free
    See all Properdin/PFC primary antibodies

  • Description

    Mouse monoclonal [6E9E6] to Properdin/PFC - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human plasma; Rat serum; Mouse plasma.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

    ab254276 is the carrier-free version of ab252532. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254276 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    6E9E6

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)
    Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)
    Anti-Properdin/PFC antibody [6E9E6] (ab252532) at 1/500 dilution + Human plasma lysate 20ug

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131368) at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab252532, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24797388). 

    Exposure time: 15 seconds.

  • Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)
    Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)
    All lanes : Anti-Properdin/PFC antibody [6E9E6] (ab252532) at 1/500 dilution

    Lane 1 : Rat serum lysate 20ug
    Lane 2 : Mouse plasma lysate 20ug

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131368) at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using ab252532, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24797388). 

    Exposure time: 70 seconds.

  • ELISA - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)
    ELISA - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)

    This data was developed using ab252532, the same antibody clone in a different buffer formulation.

    Indirect ELISA antibody dose-response curve using properdin at 1000 ng/ml and ab252532 at 0-2000 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution.

  • Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)
    Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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