Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661)
![Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661)](https://www.abcam.com/ps/products/273/ab273661/Images/ab273661-394565-anti-NR1H4-antibody-ihc-human-thyriod-carcinoma-tissue.JPG "Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661)")
Key features and details
- Mouse monoclonal [OTI4F12] to Bile Acid Receptor NR1H4 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free
See all Bile Acid Receptor NR1H4 primary antibodies -
Description
Mouse monoclonal [OTI4F12] to Bile Acid Receptor NR1H4 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Bile Acid Receptor NR1H4 aa 1-300. NP_005114 Produced in E.coli
Database link: Q96RI1-2 -
Positive control
- WB: Bile Acid Receptor NR1H4 transfected HEK-293T cell lysate; IHC-P: Human thyroid carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
OTI4F12 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661)
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue staining Bile Acid Receptor NR1H4 using ab273661 at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
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![Western blot - Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661)](https://www.abcam.com/ps/products/273/ab273661/Images/ab273661-394566-anti-NR1H4-antibody-wb-transfected-hek293t-cells.jpg)
Western blot - Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661)All lanes : Anti-Bile Acid Receptor NR1H4 antibody [OTI4F12] - BSA and Azide free (ab273661) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY Bile Acid Receptor NR1H4-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 56 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.