Anti-BHMT antibody [3D6] (ab282156)
![Anti-BHMT antibody [3D6] (ab282156)](https://www.abcam.com/ps/products/282/ab282156/Images/ab282156-2-anti-human-BHMT-antibody-3D6-wb-mouse-liver-cells.jpg "Anti-BHMT antibody [3D6] (ab282156)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3D6] to BHMT
- Suitable for: ICC, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Overview
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Product name
Anti-BHMT antibody [3D6]
See all BHMT primary antibodies -
Description
Mouse monoclonal [3D6] to BHMT -
Host species
Mouse -
Tested applications
Suitable for: ICC, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human BHMT aa 1-450.
Database link: Q93088 -
Positive control
- WB: Mouse liver tissue lysate. ICC: Hep3B cells. FC: Hep3B cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3D6 -
Isotype
IgG2a -
Light chain type
kappa
Images
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Western blot - Anti-BHMT antibody (ab282156)Anti-BHMT antibody [3D6] (ab282156) at 1/1000 dilution + Mouse liver tissue lysate at 40 µg
Secondary
Goat anti-Mouse secondary antibody HRP-conjugate
Predicted band size: 45 kDaSDS-PAGE gel was transferred to PVDF membrane.
ECL detection system.
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Immunocytochemistry - Anti-BHMT antibody (ab282156)Immunocytochemical analysis of Hep3B cells labelling BHMT with ab282156 at 1/100 dilution followed by Alexa Fluor® 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).
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Flow Cytometry - Anti-BHMT antibody (ab282156)Flow cytometry analysis of Hep3B cells labelling BHMT with ab282156 at 2-5ug for 1x106 cells (red). A Goat anti-mouse IgG (Alexa Fluor® 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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![Anti-BHMT antibody [3D6] (ab282156)](https://www.abcam.com/ps/products/282/ab282156/Images/ab282156-464272-benefitsofrecombinantantibodies.jpg)
Anti-BHMT antibody [3D6] (ab282156)
