Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BHMT antibody [3D6] (ab282156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [3D6] to BHMT
  • Suitable for: ICC, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BHMT antibody [3D6]
    See all BHMT primary antibodies

  • Description

    Mouse monoclonal [3D6] to BHMT

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein corresponding to Human BHMT aa 1-450.
    Database link: Q93088

  • Positive control

    • WB: Mouse liver tissue lysate. ICC: Hep3B cells. FC: Hep3B cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    3D6

  • Isotype

    IgG2a

  • Light chain type

    kappa

Images

  • Western blot - Anti-BHMT antibody (ab282156)
    Western blot - Anti-BHMT antibody (ab282156)
    Anti-BHMT antibody [3D6] (ab282156) at 1/1000 dilution + Mouse liver tissue lysate at 40 µg

    Secondary
    Goat anti-Mouse secondary antibody HRP-conjugate

    Predicted band size: 45 kDa



    SDS-PAGE gel was transferred to PVDF membrane.

    ECL detection system.

  • Immunocytochemistry - Anti-BHMT antibody (ab282156)
    Immunocytochemistry - Anti-BHMT antibody (ab282156)

    Immunocytochemical analysis of Hep3B cells labelling BHMT with ab282156 at 1/100 dilution followed by Alexa Fluor® 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).

  • Flow Cytometry - Anti-BHMT antibody (ab282156)
    Flow Cytometry - Anti-BHMT antibody (ab282156)

    Flow cytometry analysis of Hep3B cells labelling BHMT with ab282156 at 2-5ug for 1x106 cells (red). A Goat anti-mouse IgG (Alexa Fluor® 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

  • Anti-BHMT antibody [3D6] (ab282156)
    Anti-BHMT antibody [3D6] (ab282156)

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