Anti-beta III Tubulin (phospho S172) antibody (ab76286)
Key features and details
- Rabbit polyclonal to beta III Tubulin (phospho S172)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-beta III Tubulin (phospho S172) antibody
See all beta III Tubulin primary antibodies -
Description
Rabbit polyclonal to beta III Tubulin (phospho S172) -
Host species
Rabbit -
Specificity
The ab76286 sequence is identical to similar regions in bI, bII, and bIII tubulin isotypes. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Synthetic phospho peptide corresponding to amino acid residues around serine 172 of human beta III Tubulin conjugated to KLH
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Positive control
- C2C12 cells; purified brain tubulin treated with ERK2 kinase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76286 was cross adsorbed to unphosphorylated beta III Tubulin (Ser 172) peptide before affinity purification using phospho beta III Tubulin (Ser 172) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin (phospho S172) antibody (ab76286)ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence.
Image 1: untreated.
Image 2: in the presence of the phospho peptide. -
Western blot - Anti-beta III Tubulin (phospho S172) antibody (ab76286)All lanes : Anti-beta III Tubulin (phospho S172) antibody (ab76286) at 1/1000 dilution
Lane 1 : purified brain tubulin, untreated
Lane 2 : purified brain tubulin, treated with ERK2 kinase to phosphorylate
Ser 172
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
