Anti-TUBA1A antibody (ab95966)
Key features and details
- Rabbit polyclonal to TUBA1A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-TUBA1A antibody -
Description
Rabbit polyclonal to TUBA1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 37-286 of Human TUBA1A (NP_006000).
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Positive control
- WB: Jurkat, Raji, HEK-293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH/3T3 whole cell lysate, mouse and rat brain tissue lysat, drosophila lysate. IHC: SAS xenograft tissue. IF: HeLa whole cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 8 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 10 : NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate
Lane 11 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 12 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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Immunofluorescence analysis of 4% paraformaldehyde fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells lalbeling TUBA1A at cytoskeleton with ab95966 at 1/500 dilution. Blue: Hoechst 33342 staining.
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All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 2 : HEK-293T whole cell lysate at 5 µg
Lane 3 : HEK-293T whole cell lysate at 2 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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Anti-TUBA1A antibody (ab95966) at 1/10000 dilution + Rat brain tissue lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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Immunofluorescence analysis of 4% paraformaldehyde fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TUBA1A at cytoskeleton with ab95966 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Anti-TUBA1A antibody (ab95966) at 1/10000 dilution + Drosophila lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE