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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators

Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E109] to beta Arrestin 1 (phospho S412)
  • Suitable for: Dot blot, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-beta Arrestin 1 (phospho S412) antibody [E109]
    See all beta Arrestin 1 primary antibodies
  • Description

    Rabbit monoclonal [E109] to beta Arrestin 1 (phospho S412)
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for beta Arrestin 1 phosphorylated on Serine 412.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 412 of Human beta Arrestin 1.

  • Positive control

    • WB: 293T cell lysate. IHC-P: Human lymph node.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    E109
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • Regulators
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors

Images

  • Western blot - Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
    Western blot - Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
    Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097) at 1/20000 dilution + 293T cell lysate.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Dot Blot - Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
    Dot Blot - Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
    Ab32097, at a dilution of 1/1000, staining beta Arrestin 1 by Dot Blot.
    A nitrocellulose membrane was spotted with (1) phosphopeptide and (2) non phosphopeptide at 1 and 0.1ng and then blotted with ab32097.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)

    Ab32097, at a 1/500 dilution, staining beta Arrestin 1 in paraffin embedded human lymph node tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
    Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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