Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E109] to beta Arrestin 1 (phospho S412)
- Suitable for: Dot blot, WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-beta Arrestin 1 (phospho S412) antibody [E109]
See all beta Arrestin 1 primary antibodies -
Description
Rabbit monoclonal [E109] to beta Arrestin 1 (phospho S412) -
Host species
Rabbit -
Specificity
This antibody is specific for beta Arrestin 1 phosphorylated on Serine 412. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Serine 412 of Human beta Arrestin 1.
-
Positive control
- WB: 293T cell lysate. IHC-P: Human lymph node.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
E109 -
Isotype
IgG -
Research areas
Images
-
Anti-beta Arrestin 1 (phospho S412) antibody [E109] (ab32097) at 1/20000 dilution + 293T cell lysate.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
-
Ab32097, at a dilution of 1/1000, staining beta Arrestin 1 by Dot Blot.
A nitrocellulose membrane was spotted with (1) phosphopeptide and (2) non phosphopeptide at 1 and 0.1ng and then blotted with ab32097. -
Ab32097, at a 1/500 dilution, staining beta Arrestin 1 in paraffin embedded human lymph node tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-