Anti-beta Arrestin 1 (phospho S412) antibody (ab4472)
Key features and details
- Rabbit polyclonal to beta Arrestin 1 (phospho S412)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-beta Arrestin 1 (phospho S412) antibody
See all beta Arrestin 1 primary antibodies -
Description
Rabbit polyclonal to beta Arrestin 1 (phospho S412) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic phospho peptide (Rat) derived from the region of beta Arrestin 1 that contains Ser 412.
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Positive control
- PC12 and CHO-K cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated beta-arrestin-1 protein. The final product is generated by affinity chromatography using a beta Arrestin 1 derived peptide that is phosphorylated at serine 412. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Arrestin 1 (phospho S412) antibody (ab4472)
Peptide Competition:
Extracts prepared from PC12 cells were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with
0.75µ g/mL phospho beta Arrestin 1 (Ser 412) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-serine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.Phosphatase Stripping:
Extracts and membranes were prepared as above. The membrane was either not treated (5, 7) or treated (6, 8) with PP2A phosphatase, then incubated with beta Arrestin 1 pan antibody (5, 6), or 0.75µ g/mL phospho beta A
