Anti-beta 2 Microglobulin antibody (ab227261)
Key features and details
- Rabbit polyclonal to beta 2 Microglobulin
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-beta 2 Microglobulin antibody
See all beta 2 Microglobulin primary antibodies -
Description
Rabbit polyclonal to beta 2 Microglobulin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human beta 2 Microglobulin.
Database link: P61769 -
Positive control
- WB: Mouse and rat plasma extract; A431 whole cell extract. IHC-P: Human endometrial cancer tissue; Cal27 xenograft tissue. IP: HeLa whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human endometrial cancer tissue stained for beta 2 Microglobulin using ab227261 at 1/500 dilution in immunohistochemical analysis.
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Anti-beta 2 Microglobulin antibody (ab227261) at 1/500 dilution + Mouse plasma extract at 50 µg
Predicted band size: 14 kDa15% SDS-PAGE gel.
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beta 2 Microglobulin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg ab227261. Western blot was performed from the immunoprecipitate using ab227261 at 1/500 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract 30 μg.
Lane 1: Control IP in HeLa whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 2: ab227261 IP in HeLa whole cell extract.
15 % SDS-PAGE gel.
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Paraffin-embedded Cal27 (human) xenograft stained for beta 2 Microglobulin using ab227261 at 1/500 dilution in immunohistochemical analysis.
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Anti-beta 2 Microglobulin antibody (ab227261) at 1/500 dilution + Rat plasma extract at 50 µg
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Anti-beta 2 Microglobulin antibody (ab227261) at 1/5000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate
Predicted band size: 14 kDa15% SDS-PAGE gel.