Anti-Bcl3 antibody [EPR23835-115] (ab259832)
![Anti-Bcl3 antibody [EPR23835-115] (ab259832)](https://www.abcam.com/ps/products/259/ab259832/Images/ab259832-450348-anti-bcl3-antibody-epr23835115-western-blot-carcinoma.jpg "Anti-Bcl3 antibody [EPR23835-115] (ab259832)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23835-115] to Bcl3
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Bcl3 antibody [EPR23835-115]
See all Bcl3 primary antibodies -
Description
Rabbit monoclonal [EPR23835-115] to Bcl3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, C2C12 and HeLa whole cell lysate. Mouse ovary tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23835-115 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)All lanes : Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 2 : mouse ovary tissue lysate
Lane 3 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Fresh lysate was used in lane 1.
Exposure time: Lane 1: 5.5 seconds; Lane 2-3: 3 minutes.
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![Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)](https://www.abcam.com/ps/products/259/ab259832/Images/ab259832-450349-anti-bcl3-antibody-epr23835115-western-blot-adenocarcinoma.jpg)
Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)All lanes : Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : THP-1 (human monocytic leukemia monocyte), whole cell lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: THP-1 (PMID: 25089799).
Exposure time: 81 seconds.