Anti-Bcl10 antibody [4F8E8H8] (ab36585)
Key features and details
- Mouse monoclonal [4F8E8H8] to Bcl10
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Bcl10 antibody [4F8E8H8]
See all Bcl10 primary antibodies -
Description
Mouse monoclonal [4F8E8H8] to Bcl10 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Mouse -
Immunogen
Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).
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Positive control
- MCF-7 cell extract. Human breast and liver carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4F8E8H8 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution
Lane 1 : Cell lysates prepared from NIH/3T3 cells
Lane 2 : Cell lysates prepared from Hela cells
Lane 3 : Cell lysates prepared from MCF-7 cells
Lane 4 : Cell lysates prepared from Jurkat cells
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size: 31 kDa
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Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
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Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
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ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).