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Anti-Bcl10 antibody [4F8E8H8] (ab36585)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Bcl10 antibody [4F8E8H8] (ab36585)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4F8E8H8] to Bcl10
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Bcl10 antibody [4F8E8H8]
    See all Bcl10 primary antibodies
  • Description

    Mouse monoclonal [4F8E8H8] to Bcl10
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

  • Positive control

    • MCF-7 cell extract. Human breast and liver carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4F8E8H8
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Western blot - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution

    Lane 1 : Cell lysates prepared from NIH/3T3 cells
    Lane 2 : Cell lysates prepared from Hela cells
    Lane 3 : Cell lysates prepared from MCF-7 cells
    Lane 4 : Cell lysates prepared from Jurkat cells

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
  • Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [4F8E8H8] (ab36585)

    ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.

  • Flow Cytometry - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Flow Cytometry - Anti-Bcl10 antibody [4F8E8H8] (ab36585)
    Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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