Anti-BAZ2A/TIP5 antibody - ChIP Grade (ab195278)
Key features and details
- Rabbit polyclonal to BAZ2A/TIP5 - ChIP Grade
- Suitable for: ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BAZ2A/TIP5 antibody - ChIP Grade
See all BAZ2A/TIP5 primary antibodies -
Description
Rabbit polyclonal to BAZ2A/TIP5 - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP MouseHumanWB Mouse
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Immunogen
Recombinant fragment corresponding to Human BAZ2A/TIP5.
Database link: QU9IF9 -
Positive control
- HEK293T, NIH 3T3 and HeLa cell extacts.
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General notes
This product was previously labelled as BAZ2A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BAZ2A/TIP5 antibody - ChIP Grade (ab195278)All lanes : Anti-BAZ2A/TIP5 antibody - ChIP Grade (ab195278) at 1/1000 dilution
Lane 1 : NIH 3T3 nuclear extract
Lane 2 : HeLa nuclear extract
Lysates/proteins at 150 µg per lane.
Predicted band size: 211 kDa
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ChIP - Anti-BAZ2A/TIP5 antibody - ChIP Grade (ab195278)ChIP assays were performed using ab195278. Chromatin from HEK293T and NIH 3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 µg sheared chromatin and either 5 µl ab195278 or 5 µl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure shows the recovery by ab195278 and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH 3T3 cells, BAZ2A/TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.
