Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (ab249327)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11326(B)] to ATP6V1D - BSA and Azide free
- Suitable for: ICC, WB, IHC-P, Flow Cyt, IP
- Reacts with: Rat, Human
Overview
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Product name
Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free
See all ATP6V1D primary antibodies -
Description
Rabbit monoclonal [EPR11326(B)] to ATP6V1D - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249327 is the carrier-free version of ab157458. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249327 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11326(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab157458, the same antibody clone in a different buffer formulation.
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This data was developed using ab157458, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab157458, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab157458, the same antibody clone in a different buffer formulation.
Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.
Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution + Immunoprecipitation pellet from Y79 cell lysate at 10 µg
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