Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24276-125] to MAG/GMA - BSA and Azide free
  • Suitable for: IHC-Fr, ICC, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free
    See all MAG/GMA primary antibodies

  • Description

    Rabbit monoclonal [EPR24276-125] to MAG/GMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Rat
    IHC-Fr
    Mouse
    IHC-P
    Rat
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum tissue lysate; Mouse brain and cerebellum tissue lysates; Rat cerebellum tissue lysate. IHC-P: Human cerebellum and sciatic nerve tissue; Mouse hippocampus tissue; Rat hippocampus tissue. IHC-Fr: Mouse cerebellum tissue; Rat cerebellum tissue. ICC: Rat primary neural/glia cells. IP: Human cerebellum tissue lysate; Mouse brain tissue lysate.

  • General notes

    ab277535 is the carrier-free version of ab277524. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277535 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAG/GMA with ab277524 at 1/5000 dilution (0.1008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum.The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling MAG/GMA with ab277524 at 1/500 dilution (1.008 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunocytochemistry - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cell cells labelling MAG/GMA with ab277524 at 1/50 dilution (10.08 ug/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary oligodendroglia cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-Myelin Basic Protein rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab277241 at 1/50 dilution, followed by ab150157 at 1/1000 dilution.

    -ve control 2: Anti-Myelin Basic Protein rat monoclonal antibody, followed by ab150080 at 1/1000 dilution.

  • Western blot - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Western blot - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    All lanes : Anti-MAG/GMA antibody [EPR24276-125] (ab277524) at 1/1000 dilution

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab277524, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    MAG is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 1716323).

    Negative control: Liver (PMID: 2432614).

    Exposure time: 26 seconds.

  • Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    MAG/GMA was immunoprecipitated from 0.35 mg human cerebellum tissue lysate with ab277524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human cerebellum tissue lysate 10 ug

    Lane 2: ab277524 IP in Human cerebellum tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277524 in Human cerebellum tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 23 seconds.

     

  • Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling MAG/GMA with ab277524 at 1/500 dilution (1.008 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Western blot - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    All lanes : Anti-MAG/GMA antibody [EPR24276-125] (ab277524) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse cerebellum tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat cerebellum tissue lysate
    Lane 5 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 69 kDa



    This data was developed using ab277524, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    MAG is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 1716323).

    Negative control: Liver (PMID: 2432614).

    Exposure time: 126 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling MAG/GMA with ab277524 at 1/100 dilution (5.04 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse liver (PMID2432614) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    MAG/GMA was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab277524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab277524 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277524 in Mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 23 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human sciatic nerve tissue labeling MAG/GMA with ab277524 at 1/5000 dilution (0.1008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human sciatic nerve. The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling MAG/GMA with ab277524 at 1/100 dilution (5.04 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on rat liver (PMID2432614) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling MAG/GMA with ab277524 at 1/2000 dilution (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus. (PMID: 25523827)The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

    This data was developed using ab277524, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling MAG/GMA with ab277524 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hippocampus. (PMID: 17705198, 27000654 )The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)
    Anti-MAG/GMA antibody [EPR24276-125] - BSA and Azide free (ab277535)

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