Anti-ATG4C antibody - C-terminal (ab227610)
Key features and details
- Rabbit polyclonal to ATG4C - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG4C antibody - C-terminal
See all ATG4C primary antibodies -
Description
Rabbit polyclonal to ATG4C - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human ATG4C (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q96DT6 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATG4C antibody - C-terminal (ab227610)All lanes : Anti-ATG4C antibody - C-terminal (ab227610) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : HEK-293T transfected with ATG4C shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa10% SDS-PAGE
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Western blot - Anti-ATG4C antibody - C-terminal (ab227610)All lanes : Anti-ATG4C antibody - C-terminal (ab227610) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa10% SDS-PAGE
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Immunoprecipitation - Anti-ATG4C antibody - C-terminal (ab227610)ATG4C was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen whole cell lysate with 5 μg ab227610. Western blot was performed from the immunoprecipitate using ab227610. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab227610 IP in HEK-293T whole cell extract.
