Anti-ATG4C antibody (ab223370)
Key features and details
- Rabbit polyclonal to ATG4C
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG4C antibody
See all ATG4C primary antibodies -
Description
Rabbit polyclonal to ATG4C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human ATG4C aa 250-350.
Database link: Q96DT6 -
Positive control
- WB: HEK-293T cell lysate. ICC/IF: RKO cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add 1% BSA for extra stability. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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4% formaldehyde-fixed RKO cells stained for ATG4C (green) using ab223370 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 60 minutes at room temperature; DAPI (blue) nuclear stain at 1/5000 for 5 minutes at room temperature.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223370 (D) Merge
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Anti-ATG4C antibody (ab223370) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 6 minutesPrimary antibody was incubated for 16 hours at 4°C, the secondary for 60 minutes at room temperature.